|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Canine IL1B ORF mammalian expression plasmid, C-GFPSpark tag||DG70018-ACG|
|Canine IL1B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70018-ACR|
|Canine IL1B ORF mammalian expression plasmid, C-Flag tag||DG70018-CF|
|Canine IL1B ORF mammalian expression plasmid, C-His tag||DG70018-CH|
|Canine IL1B ORF mammalian expression plasmid, C-Myc tag||DG70018-CM|
|Canine IL1B ORF mammalian expression plasmid, C-HA tag||DG70018-CY|
|Canine IL1B Gene cDNA clone plasmid||DG70018-G|
|Canine IL1B ORF mammalian expression plasmid, N-Flag tag||DG70018-NF|
|Canine IL1B ORF mammalian expression plasmid, N-His tag||DG70018-NH|
|Canine IL1B ORF mammalian expression plasmid, N-Myc tag||DG70018-NM|
|Canine IL1B ORF mammalian expression plasmid, N-HA tag||DG70018-NY|
|Canine IL1B natural ORF mammalian expression plasmid||DG70018-UT|
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Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).