|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Canine IL21 ORF mammalian expression plasmid, C-GFPSpark tag||DG70016-ACG|
|Canine IL21 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70016-ACR|
|Canine IL21 ORF mammalian expression plasmid, C-Flag tag||DG70016-CF|
|Canine IL21 ORF mammalian expression plasmid, C-His tag||DG70016-CH|
|Canine IL21 ORF mammalian expression plasmid, C-Myc tag||DG70016-CM|
|Canine IL21 ORF mammalian expression plasmid, C-HA tag||DG70016-CY|
|Canine IL21 Gene cDNA clone plasmid||DG70016-G|
|Canine IL21 ORF mammalian expression plasmid, N-Flag tag||DG70016-NF|
|Canine IL21 ORF mammalian expression plasmid, N-His tag||DG70016-NH|
|Canine IL21 ORF mammalian expression plasmid, N-Myc tag||DG70016-NM|
|Canine IL21 ORF mammalian expression plasmid, N-HA tag||DG70016-NY|
|Canine IL21 natural ORF mammalian expression plasmid||DG70016-UT|
|Learn more about expression Vectors|
IL21 belongs to the IL-15/IL-21 family. It is a cytokine with immunoregulatory activity. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. IL21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. It may promote the transition between innate and adaptive immunity. IL-21 has been tried as therapy for alleviating allergic responses. It can significantly decrease pro-inflammatory cytokines produced by T cells in addition to decreasing IgE levels in a mouse model for rhinitis (nasal passage inflammation)