|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Canine VEGFA ORF mammalian expression plasmid, C-GFPSpark tag||DG70004-ACG|
|Canine VEGFA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70004-ACR|
|Canine VEGFA ORF mammalian expression plasmid, C-Flag tag||DG70004-CF|
|Canine VEGFA ORF mammalian expression plasmid, C-His tag||DG70004-CH|
|Canine VEGFA ORF mammalian expression plasmid, C-Myc tag||DG70004-CM|
|Canine VEGFA ORF mammalian expression plasmid, C-HA tag||DG70004-CY|
|Canine VEGFA Gene cDNA clone plasmid||DG70004-G|
|Canine VEGFA ORF mammalian expression plasmid, N-Flag tag||DG70004-NF|
|Canine VEGFA ORF mammalian expression plasmid, N-His tag||DG70004-NH|
|Canine VEGFA ORF mammalian expression plasmid, N-Myc tag||DG70004-NM|
|Canine VEGFA ORF mammalian expression plasmid, N-HA tag||DG70004-NY|
|Canine VEGFA natural ORF mammalian expression plasmid||DG70004-UT|
|Learn more about expression Vectors|
Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.