|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rhesus ADAM8 ORF mammalian expression plasmid, C-GFPSpark tag||CG90278-ACG|
|Rhesus ADAM8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90278-ACR|
|Rhesus ADAM8 ORF mammalian expression plasmid, C-Flag tag||CG90278-CF|
|Rhesus ADAM8 ORF mammalian expression plasmid, C-His tag||CG90278-CH|
|Rhesus ADAM8 ORF mammalian expression plasmid, C-Myc tag||CG90278-CM|
|Rhesus ADAM8 ORF mammalian expression plasmid, C-HA tag||CG90278-CY|
|Rhesus ADAM8 Gene cDNA clone plasmid||CG90278-G|
|Rhesus ADAM8 ORF mammalian expression plasmid, N-Flag tag||CG90278-NF|
|Rhesus ADAM8 ORF mammalian expression plasmid, N-His tag||CG90278-NH|
|Rhesus ADAM8 ORF mammalian expression plasmid, N-Myc tag||CG90278-NM|
|Rhesus ADAM8 ORF mammalian expression plasmid, N-HA tag||CG90278-NY|
|Rhesus ADAM8 natural ORF mammalian expression plasmid||CG90278-UT|
|Learn more about expression Vectors|
ADAM8, also known as CD156, is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. ADAM8 is possiblely involved in extravasation of leukocytes As a metalloprotease, ADAM8 also may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma.