|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rhesus CD247 ORF mammalian expression plasmid, C-GFPSpark tag||CG90248-ACG|
|Rhesus CD247 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90248-ACR|
|Rhesus CD247 ORF mammalian expression plasmid, C-Flag tag||CG90248-CF|
|Rhesus CD247 ORF mammalian expression plasmid, C-His tag||CG90248-CH|
|Rhesus CD247 ORF mammalian expression plasmid, C-Myc tag||CG90248-CM|
|Rhesus CD247 ORF mammalian expression plasmid, C-HA tag||CG90248-CY|
|Rhesus CD247 Gene cDNA clone plasmid||CG90248-G|
|Rhesus CD247 ORF mammalian expression plasmid, N-Flag tag||CG90248-NF|
|Rhesus CD247 ORF mammalian expression plasmid, N-His tag||CG90248-NH|
|Rhesus CD247 ORF mammalian expression plasmid, N-Myc tag||CG90248-NM|
|Rhesus CD247 ORF mammalian expression plasmid, N-HA tag||CG90248-NY|
|Rhesus CD247 natural ORF mammalian expression plasmid||CG90248-UT|
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CD247, also known as CD3-ZETA, belongs to the CD3Z/FCER1G family. It contains 3 ITAM domains. As a -cell receptor zeta, CD247 forms the T-cell receptor-CD3 complex together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and –epsilon. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for CD247 gene. Defects in CD247 can cause immunodeficiency due to defect in CD3-zeta. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. CD247 may play a role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.