|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CD44 ORF mammalian expression plasmid, C-GFPSpark tag||HG12211-ACG|
|Human CD44 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12211-ACR|
|Human CD44 ORF mammalian expression plasmid, C-Flag tag||HG12211-CF|
|Human CD44 ORF mammalian expression plasmid, C-His tag||HG12211-CH|
|Human CD44 ORF mammalian expression plasmid, C-Myc tag||HG12211-CM|
|Human CD44 ORF mammalian expression plasmid, C-HA tag||HG12211-CY|
|Human CD44 Gene cDNA clone plasmid||HG12211-G|
|Human CD44 ORF mammalian expression plasmid, N-Flag tag||HG12211-NF|
|Human CD44 ORF mammalian expression plasmid, N-His tag||HG12211-NH|
|Human CD44 ORF mammalian expression plasmid, N-Myc tag||HG12211-NM|
|Human CD44 ORF mammalian expression plasmid, N-HA tag||HG12211-NY|
|Human CD44 natural ORF mammalian expression plasmid||HG12211-UT|
|Learn more about expression Vectors|
CD44 is a type I transmembrane protein and a member of the cartilage link protein family. It is involved in cell-cell and cell-matrix interactions and signal transduction. Several CD44 ligands have been identified. The most extensively characterized ligand for CD44 is hyaluronan, a component of the extracellular matrix. CD44 protein is expressed on the majority of immune cells. The binding of CD44 to hyaluronan is induced on T lymphocytes after activation by antigen and on monocytes after stimulation by inflammatory agents. Under inflammatory conditions, CD44 on endothelial cells presents hyaluronan to CD44 on activated T lymphocytes and mediates a rolling interaction under flow conditions. Perturbations of the hyaluronan-CD44 interaction at the plasma membrane by various antagonists result in attenuation of receptor tyrosine kinase and transporter activities and inhibition of tumor progression in vivo. CD44 is known to interact with the ezrin family (ERM family) members and form a complex that plays diverse roles within both normal and abnormal cells, particularly cancer cells. CD44 and ezrin and their respective complex have properties suggesting that they may be important in the process of tumour-endothelium interactions, cell migrations, cell adhesion, tumour progression and metastasis.