|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GCSH ORF mammalian expression plasmid, C-GFPSpark tag||HG14568-ACG|
|Human GCSH ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14568-ACR|
|Human GCSH ORF mammalian expression plasmid, N-GFPSpark tag||HG14568-ANG|
|Human GCSH ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14568-ANR|
|Human GCSH ORF mammalian expression plasmid, C-Flag tag||HG14568-CF|
|Human GCSH ORF mammalian expression plasmid, C-His tag||HG14568-CH|
|Human GCSH ORF mammalian expression plasmid, C-Myc tag||HG14568-CM|
|Human GCSH ORF mammalian expression plasmid, C-HA tag||HG14568-CY|
|Human GCSH Gene cDNA clone plasmid||HG14568-G|
|Human GCSH ORF mammalian expression plasmid, N-Flag tag||HG14568-NF|
|Human GCSH ORF mammalian expression plasmid, N-His tag||HG14568-NH|
|Human GCSH ORF mammalian expression plasmid, N-Myc tag||HG14568-NM|
|Human GCSH ORF mammalian expression plasmid, N-HA tag||HG14568-NY|
|Human GCSH natural ORF mammalian expression plasmid||HG14568-UT|
|Learn more about expression Vectors|
Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). GCSH is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in GCSH gene are a cause of nonketotic hyperglycinemia (NKH). Two transcript variants, one protein-coding and the other probably not protein-coding,have been found for GCSH gene. Also, several transcribed and non-transcribed pseudogenes of GCSH gene exist throughout the genome.