|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GUCA1A ORF mammalian expression plasmid, C-GFPSpark tag||HG14565-ACG|
|Human GUCA1A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14565-ACR|
|Human GUCA1A ORF mammalian expression plasmid, C-Flag tag||HG14565-CF|
|Human GUCA1A ORF mammalian expression plasmid, C-His tag||HG14565-CH|
|Human GUCA1A ORF mammalian expression plasmid, C-Myc tag||HG14565-CM|
|Human GUCA1A ORF mammalian expression plasmid, C-HA tag||HG14565-CY|
|Human GUCA1A Gene cDNA clone plasmid||HG14565-G|
|Human GUCA1A ORF mammalian expression plasmid, N-Flag tag||HG14565-NF|
|Human GUCA1A ORF mammalian expression plasmid, N-His tag||HG14565-NH|
|Human GUCA1A ORF mammalian expression plasmid, N-Myc tag||HG14565-NM|
|Human GUCA1A ORF mammalian expression plasmid, N-HA tag||HG14565-NY|
|Human GUCA1A natural ORF mammalian expression plasmid||HG14565-UT|
|Learn more about expression Vectors|
GCAP 1 gene plays a role in the recovery of retinal photoreceptors from photobleaching. In the recovery phase, the phototransduction messeneger cGMP is replenished by retinal guanylyl cyclase-1 (GC1). GC1 is activated by decreasing Ca(2+) concentrations following photobleaching. The protein encoded by this gene, guanylyl cyclase activating protein 1 (GCAP 1), mediates the sensitivity of GC1 to Ca(2+) concentrations. GCAP 1 promotes activity of GC1 at low Ca(2+) concentrations and inhibits GC1 activity at high Ca(2+) concentrations. Mutations in GCAP 1 gene cause autosomal dominant cone dystrophy (COD3); a disease characterized by reduced visual acuity associated with progressive loss of color vision. GCAP 1 stimulates guanylyl cyclase 1 (GC1) when free calcium ions concentration is low and inhibits GC1 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure.