|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rhesus CD1B ORF mammalian expression plasmid, C-GFPSpark tag||CG90173-ACG|
|Rhesus CD1B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90173-ACR|
|Rhesus CD1B ORF mammalian expression plasmid, C-Flag tag||CG90173-CF|
|Rhesus CD1B ORF mammalian expression plasmid, C-His tag||CG90173-CH|
|Rhesus CD1B ORF mammalian expression plasmid, C-Myc tag||CG90173-CM|
|Rhesus CD1B ORF mammalian expression plasmid, C-HA tag||CG90173-CY|
|Rhesus CD1B Gene cDNA clone plasmid||CG90173-G|
|Rhesus CD1B ORF mammalian expression plasmid, N-Flag tag||CG90173-NF|
|Rhesus CD1B ORF mammalian expression plasmid, N-His tag||CG90173-NH|
|Rhesus CD1B ORF mammalian expression plasmid, N-Myc tag||CG90173-NM|
|Rhesus CD1B ORF mammalian expression plasmid, N-HA tag||CG90173-NY|
|Rhesus CD1B natural ORF mammalian expression plasmid||CG90173-UT|
|Learn more about expression Vectors|
CD1B contains 1 Ig-like (immunoglobulin-like) domain and belongs to the CD1 family. CD1 family members are transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. During protein synthesis and maturation, they bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface. CD1B localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens.. It is expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues. CD1B is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.