|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PDZD11 ORF mammalian expression plasmid, C-GFPSpark tag||HG12188-ACG|
|Human PDZD11 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12188-ACR|
|Human PDZD11 ORF mammalian expression plasmid, C-Flag tag||HG12188-CF|
|Human PDZD11 ORF mammalian expression plasmid, C-His tag||HG12188-CH|
|Human PDZD11 ORF mammalian expression plasmid, C-Myc tag||HG12188-CM|
|Human PDZD11 ORF mammalian expression plasmid, C-HA tag||HG12188-CY|
|Human PDZD11 Gene cDNA clone plasmid||HG12188-G|
|Human PDZD11 ORF mammalian expression plasmid, N-Flag tag||HG12188-NF|
|Human PDZD11 ORF mammalian expression plasmid, N-His tag||HG12188-NH|
|Human PDZD11 ORF mammalian expression plasmid, N-Myc tag||HG12188-NM|
|Human PDZD11 ORF mammalian expression plasmid, N-HA tag||HG12188-NY|
|Human PDZD11 natural ORF mammalian expression plasmid||HG12188-UT|
|Learn more about expression Vectors|
PDZ domain-containing protein 11, also known as AIPP1a, PISP, PDZD11 and PDZK11, is a cytosolic protein which contains one PDZ (DHR) domain. PDZD11 bears resemblance to members of the MALS / VELIS family of proteins. It contains but one PDZ domain that apparently interacts with the C-terminus of partner proteins. PDZD11 is ubiquitously expressed, and appears to target calcium and copper ATPases to basolateral cell membranes. PDZD11 is a transiently interacting partner of the PMCA b-splice forms that may play a role in their sorting to or from the plasma membrane. Full-length human PDZD11 shares 97% amino acids (aa) identity with mouse PDZD11.