|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TNFRSF21 ORF mammalian expression plasmid, C-GFPSpark tag||HG12171-ACG|
|Human TNFRSF21 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12171-ACR|
|Human TNFRSF21 ORF mammalian expression plasmid, C-Flag tag||HG12171-CF|
|Human TNFRSF21 ORF mammalian expression plasmid, C-His tag||HG12171-CH|
|Human TNFRSF21 ORF mammalian expression plasmid, C-Myc tag||HG12171-CM|
|Human TNFRSF21 ORF mammalian expression plasmid, C-HA tag||HG12171-CY|
|Human TNFRSF21 Gene cDNA clone plasmid||HG12171-G|
|Human TNFRSF21 ORF mammalian expression plasmid, N-Flag tag||HG12171-NF|
|Human TNFRSF21 ORF mammalian expression plasmid, N-His tag||HG12171-NH|
|Human TNFRSF21 ORF mammalian expression plasmid, N-Myc tag||HG12171-NM|
|Human TNFRSF21 ORF mammalian expression plasmid, N-HA tag||HG12171-NY|
|Human TNFRSF21 natural ORF mammalian expression plasmid||HG12171-UT|
|Learn more about expression Vectors|
TNFRSF21 (death receptor-6, DR6) is an orphan TNF receptor superfamily member and belongs to a subgroup of receptors called death receptors. This type I transmembrane receptor possesses four extracellular cysteine-rich motifs and a cytoplasmic death domain. DR6 is an extensively posttranslationally modified transmembrane protein and that N- and O-glycosylations of amino acids in its extracellular part. DR6 interacts with the adaptor protein TRADD and mediates signal transduction through its death domain, and expression of DR6 in mammalian cells induces activation of both NF-kappaB and JNK and cell apoptosis. DR6 knockout mice have enhanced CD4+ T cell proliferation and Th2 cytokine production, suggested that DR6 serves as an important regulatory molecule in T-helper cell activation, and is involved in inflammation and immune regulation. DR6 is expressed ubiquitously with high expression in lymphoid organs, heart, brain and pancreas. Some tumor cells overexpress DR6, typically in conjunction with elevated anti-apoptosis molecules. DR6 may also be involved in tumor cell survival and immune evasion, which is subject to future investigations.