|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CHI3L2 ORF mammalian expression plasmid, C-GFPSpark tag||HG12162-ACG|
|Human CHI3L2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12162-ACR|
|Human CHI3L2 ORF mammalian expression plasmid, C-Flag tag||HG12162-CF|
|Human CHI3L2 ORF mammalian expression plasmid, C-His tag||HG12162-CH|
|Human CHI3L2 ORF mammalian expression plasmid, C-Myc tag||HG12162-CM|
|Human CHI3L2 ORF mammalian expression plasmid, C-HA tag||HG12162-CY|
|Human CHI3L2 Gene cDNA clone plasmid||HG12162-G|
|Human CHI3L2 ORF mammalian expression plasmid, N-Flag tag||HG12162-NF|
|Human CHI3L2 ORF mammalian expression plasmid, N-His tag||HG12162-NH|
|Human CHI3L2 ORF mammalian expression plasmid, N-Myc tag||HG12162-NM|
|Human CHI3L2 ORF mammalian expression plasmid, N-HA tag||HG12162-NY|
|Human CHI3L2 natural ORF mammalian expression plasmid||HG12162-UT|
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Chondrocyte protein 39 (YKL-39), also known as Chitinase 3-like 2 (CHI3L2), is a secretory protein of articular chondrocytes belonging to the glycosyl hydrolase 18 family. It highest expression is in chondrocytes, followed by synoviocytes, lung and heart. YKL-39/CHI3L2 is not detected in spleen, pancreas, and liver. YKL-39/CHI3L2 may also be expressed in developing brain and placenta. YKL-39/CHI3L2, a cartilage-related protein, is found to induce arthritis accompanied by pathologic changes in bone and cartilage. A better understanding of the immune response against cartilage-related components including YKL-39 may help to elucidate the pathological processes of arthritic disorders. Up regulation of YKL-39/CHI3L2 in osteoarthritic cartilage suggests that YKL-39/CHI3L2 may be a more accurate marker of chondrocyte activation than YKL-40, although it has yet to be established as a suitable marker in synovial fluid and serum. The decreased expression of YKL-40 by osteoarthritic chondrocytes is surprising as increased levels have been reported in rheumatoid and osteoarthritic synovial fluid, where it may derive from activated synovial cells or osteophytic tissue or by increased matrix destruction in the osteoarthritic joint. YKL-39 and YKL-40 are potentially interesting marker molecules for arthritic joint disease because they are abundantly expressed by both normal and osteoarthritic chondrocytes.