|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CPLX3 ORF mammalian expression plasmid, C-GFPSpark tag||HG14518-ACG|
|Human CPLX3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14518-ACR|
|Human CPLX3 ORF mammalian expression plasmid, N-GFPSpark tag||HG14518-ANG|
|Human CPLX3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14518-ANR|
|Human CPLX3 ORF mammalian expression plasmid, C-Flag tag||HG14518-CF|
|Human CPLX3 ORF mammalian expression plasmid, C-His tag||HG14518-CH|
|Human CPLX3 ORF mammalian expression plasmid, C-Myc tag||HG14518-CM|
|Human CPLX3 ORF mammalian expression plasmid, C-HA tag||HG14518-CY|
|Human CPLX3 Gene cDNA clone plasmid||HG14518-G|
|Human CPLX3 ORF mammalian expression plasmid, N-Flag tag||HG14518-NF|
|Human CPLX3 ORF mammalian expression plasmid, N-His tag||HG14518-NH|
|Human CPLX3 ORF mammalian expression plasmid, N-Myc tag||HG14518-NM|
|Human CPLX3 ORF mammalian expression plasmid, N-HA tag||HG14518-NY|
|Human CPLX3 natural ORF mammalian expression plasmid||HG14518-UT|
|Learn more about expression Vectors|
CPLX3, also known as complexin 3, belongs to the complexin/synaphin family. As a SNARE-binding protein, complexin (CPX), can act either as a facilitator or as an inhibitor of membrane fusion, constituting a controversial dilemma. CPX acts sequentially on assembling SNAREpins, first facilitating zippering by nearly doubling the distance at which v- and t-SNAREs can engage and then clamping them into a half-zippered fusion-incompetent state. Specifically, the central helix of CPX allows SNAREs to form this intermediate energetic state at 9-15 nm but not when the bilayers are closer than 9 nm. Stabilizing the activated-clamped state at separations of less than 9 nm requires the accessory helix of CPX, which prevents membrane-proximal assembly of SNAREpins. CPLX3 binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A.