|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TIMD4 ORF mammalian expression plasmid, C-GFPSpark tag||HG12161-ACG|
|Human TIMD4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12161-ACR|
|Human TIMD4 ORF mammalian expression plasmid, C-Flag tag||HG12161-CF|
|Human TIMD4 ORF mammalian expression plasmid, C-His tag||HG12161-CH|
|Human TIMD4 ORF mammalian expression plasmid, C-Myc tag||HG12161-CM|
|Human TIMD4 ORF mammalian expression plasmid, C-HA tag||HG12161-CY|
|Human TIMD4 Gene cDNA clone plasmid||HG12161-G|
|Human TIMD4 ORF mammalian expression plasmid, N-Flag tag||HG12161-NF|
|Human TIMD4 ORF mammalian expression plasmid, N-His tag||HG12161-NH|
|Human TIMD4 ORF mammalian expression plasmid, N-Myc tag||HG12161-NM|
|Human TIMD4 ORF mammalian expression plasmid, N-HA tag||HG12161-NY|
|Human TIMD4 natural ORF mammalian expression plasmid||HG12161-UT|
|Learn more about expression Vectors|
A type I transmembrane protein called TIM4 (T-cell immunoglobulin- and mucin-domain-containing molecule; also known as TIMD4), which belongs to the immunoglobulin superfamily and TIM family. TIM4 is involved in regulating T-cell proliferation and lymphotoxin signaling. It is a ligand for HAVCR1/TIMD1. Recent reports indicate that dendritic cell (DC)-derived T-cell immunoglobulin and mucin domain molecule (TIM)-4, which is expressed on dendritic cells and macrophages, plays an important role in the initiation of T(H)2 polarization. TIM4 bound apoptotic cells by recognizing phosphatidylserine via its immunoglobulin domain. The expression of TIM4 in fibroblasts enhanced their ability to engulf apoptotic cells. TIM4 is phosphatidylserine receptor for the engulfment of apoptotic cells, and may also be involved in intercellular signalling in which exosomes are involved. Modulation of TIM4 production in dendritic cells (DCs) represents a novel therapeutic approach for the treatment of peanut allergy. The interaction of TIM1/TIM4 played a critical role in sustaining the polarization status of Th2 cells in allergic rhinitis (AR) patients. Cross-linking FcgammaRI by antigen/IgG complexes increased the production of TIM4 by dendritic cells via upregulating tumor necrosis factor-alpha in DCs. Specific immunotherapy (SIT) suppresses the skewed Th2 responses via disrupting the interaction of TIM1/TIM4 in antigen-specific Th2 cells.