|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human KIAA0101 ORF mammalian expression plasmid, C-GFPSpark tag||HG14486-ACG|
|Human KIAA0101 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14486-ACR|
|Human KIAA0101 ORF mammalian expression plasmid, N-GFPSpark tag||HG14486-ANG|
|Human KIAA0101 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14486-ANR|
|Human KIAA0101 ORF mammalian expression plasmid, C-Flag tag||HG14486-CF|
|Human KIAA0101 ORF mammalian expression plasmid, C-His tag||HG14486-CH|
|Human KIAA0101 ORF mammalian expression plasmid, C-Myc tag||HG14486-CM|
|Human KIAA0101 ORF mammalian expression plasmid, C-HA tag||HG14486-CY|
|Human KIAA0101 Gene cDNA clone plasmid||HG14486-G|
|Human KIAA0101 ORF mammalian expression plasmid, N-Flag tag||HG14486-NF|
|Human KIAA0101 ORF mammalian expression plasmid, N-His tag||HG14486-NH|
|Human KIAA0101 ORF mammalian expression plasmid, N-Myc tag||HG14486-NM|
|Human KIAA0101 ORF mammalian expression plasmid, N-HA tag||HG14486-NY|
|Human KIAA0101 natural ORF mammalian expression plasmid||HG14486-UT|
|Learn more about expression Vectors|
KIAA0101, also known as p15(PAF), is a proliferating cell nuclear antigen-associated factor which interacts with proliferating cell nuclear antigen(PCNA). It was initially isolated in a yeast two-hybrid screen for PCNA binding partners, and was shown to bind PCNA competitively with the cell cycle regulator p21(WAF). KIAA0101 is localized primarily in the nucleus. It shares the conserved PCNA binding motif with several other PCNA binding proteins including CDK inhibitor p21 . KIAA0101 is involved in cell proliferation and plays a role in early tumor recurrence (ETR), and prognosis of hepatocellular carcinoma (HCC). KIAA0101 is expressed predominantly in liver, pancreas and placenta. It cannot be detected in heart or brain. It is highly expressed in a number of tumors, especially esophageal tumors, in anaplastic thyroid carcinomas and in non-small-cell lung cancer lines. Overexpression of KIAA0101 predicts high stage, early tumor recurrence, and poor prognosis of hepatocellular carcinoma. It also may be involved in protection of cells from UV-induced cell death.