|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ERH ORF mammalian expression plasmid, C-GFPSpark tag||HG14483-ACG|
|Human ERH ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14483-ACR|
|Human ERH ORF mammalian expression plasmid, N-GFPSpark tag||HG14483-ANG|
|Human ERH ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14483-ANR|
|Human ERH ORF mammalian expression plasmid, C-Flag tag||HG14483-CF|
|Human ERH ORF mammalian expression plasmid, C-His tag||HG14483-CH|
|Human ERH ORF mammalian expression plasmid, C-Myc tag||HG14483-CM|
|Human ERH ORF mammalian expression plasmid, C-HA tag||HG14483-CY|
|Human ERH Gene cDNA clone plasmid||HG14483-G|
|Human ERH ORF mammalian expression plasmid, N-Flag tag||HG14483-NF|
|Human ERH ORF mammalian expression plasmid, N-His tag||HG14483-NH|
|Human ERH ORF mammalian expression plasmid, N-Myc tag||HG14483-NM|
|Human ERH ORF mammalian expression plasmid, N-HA tag||HG14483-NY|
|Human ERH natural ORF mammalian expression plasmid||HG14483-UT|
|Learn more about expression Vectors|
ERH(enhancer of rudimentary homolog) belongs to the E(R) family. It is expressed in all tissues examined. The monomeric structure of ERH comprises a single domain consisting of three α-helices and four β-strands, which is a novel fold. In the crystal structure, ERH assumes a dimeric structure, through interactions between the β-sheet regions. The formation of an ERH dimer is consistent with the results of analytical ultracentrifugation. ERH may have a role in the cell cycle. The Drosophila protein ERH is a small protein of 104 amino acids. It has been found to be an enhancer of the rudimentary gene, involved in pyrimidine biosynthesis. From an evolutionary point of view, ERH is highly conserved and has been found to exist in probably all multicellular eukaryotic organisms. ERH interacts with POLDIP3.