|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Cynomolgus monkey TNFSF10 ORF mammalian expression plasmid, C-GFPSpark tag||CG90098-ACG|
|Cynomolgus monkey TNFSF10 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90098-ACR|
|Cynomolgus monkey TNFSF10 ORF mammalian expression plasmid, C-Flag tag||CG90098-CF|
|Cynomolgus monkey TNFSF10 ORF mammalian expression plasmid, C-His tag||CG90098-CH|
|Cynomolgus monkey TNFSF10 ORF mammalian expression plasmid, C-Myc tag||CG90098-CM|
|Cynomolgus monkey TNFSF10 ORF mammalian expression plasmid, C-HA tag||CG90098-CY|
|Cynomolgus monkey TNFSF10 Gene cDNA clone plasmid||CG90098-G|
|Cynomolgus monkey TNFSF10 ORF mammalian expression plasmid, N-Flag tag||CG90098-NF|
|Cynomolgus monkey TNFSF10 ORF mammalian expression plasmid, N-His tag||CG90098-NH|
|Cynomolgus monkey TNFSF10 ORF mammalian expression plasmid, N-Myc tag||CG90098-NM|
|Cynomolgus monkey TNFSF10 ORF mammalian expression plasmid, N-HA tag||CG90098-NY|
|Cynomolgus monkey TNFSF10 natural ORF mammalian expression plasmid||CG90098-UT|
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Tumor necrosis factor ligand superfamily member 10 (TNFSF10), also known as TNF-related apoptosis-inducing ligand (TRAIL), Apo-2 ligand, and CD253, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TNFSF10 / Apo-2L / CD253 functions as a ligand that induces the process of cell death called apoptosis. TNFSF10 / TRAIL shows homology to other members of the tumor necrosis factor superfamily. As one member of the cluster of differentiation system, TNFSF10 / CD253 is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion TNFSF10 / Apo-2L / CD253 / TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A / TRAILR1, TNFRSF10B / TRAILR2, TNFRSF10C / TRAILR3, TNFRSF10D / TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TNFSF10 / TRAIL may be modulated by binding to the decoy receptors TNFRSF10C / TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8 / JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.