|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PAH ORF mammalian expression plasmid, C-GFPSpark tag||HG12081-ACG|
|Human PAH ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG12081-ACR|
|Human PAH ORF mammalian expression plasmid, N-GFPSpark tag||HG12081-ANG|
|Human PAH ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG12081-ANR|
|Human PAH ORF mammalian expression plasmid, C-Flag tag||HG12081-CF|
|Human PAH ORF mammalian expression plasmid, C-His tag||HG12081-CH|
|Human PAH ORF mammalian expression plasmid, C-Myc tag||HG12081-CM|
|Human PAH ORF mammalian expression plasmid, C-HA tag||HG12081-CY|
|Human PAH Gene cDNA clone plasmid||HG12081-G|
|Human PAH ORF mammalian expression plasmid, N-Flag tag||HG12081-NF|
|Human PAH ORF mammalian expression plasmid, N-His tag||HG12081-NH|
|Human PAH ORF mammalian expression plasmid, N-Myc tag||HG12081-NM|
|Human PAH ORF mammalian expression plasmid, N-HA tag||HG12081-NY|
|Human PAH Gene cDNA clone plasmid||HG12081-U|
|Human PAH natural ORF mammalian expression plasmid||HG12081-UT|
|Learn more about expression Vectors|
PAH (phenylalanine hydroxylase), also known as PH, belongs to the biopterin-dependent aromatic amino acid hydroxylase family. It contains 1 ACT domain, N-terminal region of PAH is thought to contain allosteric binding sites for phenylalanine and to constitute an "inhibitory" domain that regulates the activity of a catalytic domain in the C-terminal portion of the molecule. In humans, PAH is expressed both in the liver and the kidney, and there is some indication that it may be differentially regulated in these tissues. PAH catalyzes the hydroxylation of the aromatic side-chain of phenylalanine to generate tyrosine. It is one of three members of the pterin-dependent amino acid hydroxylases, a class of monooxygenase that uses tetrahydrobiopterin and a non-heme iron for catalysis. Defects in PAH are the cause of phenylketonuria (PKU). PKU is an autosomal recessive inborn error of phenylalanine metabolism, due to severe phenylalanine hydroxylase deficiency. It is characterized by blood concentrations of phenylalanine persistently above 1200 mumol.