|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human HSPA1A ORF mammalian expression plasmid, C-GFPSpark tag||HG11660-ACG|
|Human HSPA1A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11660-ACR|
|Human HSPA1A ORF mammalian expression plasmid, N-GFPSpark tag||HG11660-ANG|
|Human HSPA1A ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11660-ANR|
|Human HSPA1A ORF mammalian expression plasmid, C-Flag tag||HG11660-CF|
|Human HSPA1A ORF mammalian expression plasmid, C-His tag||HG11660-CH|
|Human HSPA1A ORF mammalian expression plasmid, C-Myc tag||HG11660-CM|
|Human HSPA1A ORF mammalian expression plasmid, C-HA tag||HG11660-CY|
|Human HSPA1A Gene cDNA clone plasmid||HG11660-M|
|Human HSPA1A natural ORF mammalian expression plasmid||HG11660-M-N|
|Human HSPA1A ORF mammalian expression plasmid, N-Flag tag||HG11660-NF|
|Human HSPA1A ORF mammalian expression plasmid, N-His tag||HG11660-NH|
|Human HSPA1A ORF mammalian expression plasmid, N-Myc tag||HG11660-NM|
|Human HSPA1A ORF mammalian expression plasmid, N-HA tag||HG11660-NY|
|Human HSPA1A natural ORF mammalian expression plasmid||HG11660-UT|
|Learn more about expression Vectors|
HSPA1A is a member of the Hsp70 protein family. The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. HSP are abundant and conserved proteins present in all cells. Upon temperature shock or other stress stimuli, HSP are synthesized intracellularly, which may protect cells from protein denaturation or from death. Extracellularly, HSP can serve a cytokine function to initiate both innate and adaptive immunity through activation of APC. HSP serves also a chaperone function and facilitates presentation of antigen peptide to T cells. Molecular chaperones of the Hsp70 family have diverse functions in cells. They assist the folding of newly synthesized and stress-denatured proteins, as well as the import of proteins into organelles, and the dissociation of aggregated proteins. The well-conserved Hsp70 chaperones are ATP dependent: binding and hydrolysis of ATP regulates their interactions with unfolded polypeptide substrates, and ATPase cycling is necessary for their function. All cellular functions of Hsp70 chaperones use the same mechanism of ATP-driven polypeptide binding and release.