|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SUB1 ORF mammalian expression plasmid, C-GFPSpark tag||HG14326-ACG|
|Human SUB1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14326-ACR|
|Human SUB1 ORF mammalian expression plasmid, N-GFPSpark tag||HG14326-ANG|
|Human SUB1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14326-ANR|
|Human SUB1 ORF mammalian expression plasmid, C-Flag tag||HG14326-CF|
|Human SUB1 ORF mammalian expression plasmid, C-His tag||HG14326-CH|
|Human SUB1 ORF mammalian expression plasmid, C-Myc tag||HG14326-CM|
|Human SUB1 ORF mammalian expression plasmid, C-HA tag||HG14326-CY|
|Human SUB1 Gene cDNA clone plasmid||HG14326-G|
|Human SUB1 ORF mammalian expression plasmid, N-Flag tag||HG14326-NF|
|Human SUB1 ORF mammalian expression plasmid, N-His tag||HG14326-NH|
|Human SUB1 ORF mammalian expression plasmid, N-Myc tag||HG14326-NM|
|Human SUB1 ORF mammalian expression plasmid, N-HA tag||HG14326-NY|
|Human SUB1 natural ORF mammalian expression plasmid||HG14326-UT|
|Learn more about expression Vectors|
SUB1 belongs to the transcriptional coactivator PC4 family. It is a general coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. SUB1 binds single-stranded DNA. Single-stranded DNA-binding proteins play many roles in nucleic acid metabolism, but their importance during transcription remains unclear. SUB1 exhibits strong genetic interactions with factors necessary for promoter melting. It localizes near the transcription bubble in vitro and binds to promoters in vivo dependent upon preinitiation complexes assembly. SUB1 interacts with the nontemplate strand of RNApII complexes during initiation. It may also be involved in stabilizing the multiprotein transcription complex.