|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IDO1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11650-ACG|
|Human IDO1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11650-ACR|
|Human IDO1 ORF mammalian expression plasmid, N-GFPSpark tag||HG11650-ANG|
|Human IDO1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11650-ANR|
|Human IDO1 ORF mammalian expression plasmid, C-Flag tag||HG11650-CF|
|Human IDO1 ORF mammalian expression plasmid, C-His tag||HG11650-CH|
|Human IDO1 ORF mammalian expression plasmid, C-Myc tag||HG11650-CM|
|Human IDO1 ORF mammalian expression plasmid, C-HA tag||HG11650-CY|
|Human IDO1 Gene cDNA clone plasmid||HG11650-M|
|Human IDO1 ORF mammalian expression plasmid, N-Flag tag||HG11650-NF|
|Human IDO1 ORF mammalian expression plasmid, N-His tag||HG11650-NH|
|Human IDO1 ORF mammalian expression plasmid, N-Myc tag||HG11650-NM|
|Human IDO1 ORF mammalian expression plasmid, N-HA tag||HG11650-NY|
|Human IDO1 natural ORF mammalian expression plasmid||HG11650-UT|
|Learn more about expression Vectors|
Indoleamine 2,3-dioxygenase-1, also known as Indoleamine-pyrrole 2,3-dioxygenase, IDO1 and IDO, is a member of the indoleamine 2,3-dioxygenase family. IDO1 / IDO and tryptophan 2,3-dioxygenase (TDO) are tryptophan-degrading enzymes that catalyze the first step in tryptophan catabolism via the kynurenine pathway. TDO is widely distributed in both eukaryotes and bacteria. In contrast, IDO has been found only in mammals and yeast. In 2007, a third enzyme, indoleamine 2,3-dioxygenase-2 (IDO2), was discovered. IDO2 is found not only in mammals but also in lower vertebrates. IDO1 / IDO is an immunosuppressive molecule inducible in various cells. IDO1 / IDO catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. It mediates oxidative cleavage of tryptophan, an amino acid essential for cell proliferation and survival. IDO1 / IDO inhibition is proposed to have therapeutic potential in immunodeficiency-associated abnormalities, including cancer. The IDO pathway is activated in multiple tumor types. Selective inhibition of IDO1 may represent an attractive cancer therapeutic strategy via up-regulation of cellular immunity. IDO1 / IDO is an enzyme that suppresses adaptive T-cell immunity by catabolizing tryptophan from the cellular microenvironment. Inhibition of IDO pathway might enhance the efficacy of immunotherapeutic strategies for cancer.