|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ADM ORF mammalian expression plasmid, C-GFPSpark tag||HG11646-ACG|
|Human ADM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11646-ACR|
|Human ADM ORF mammalian expression plasmid, C-Flag tag||HG11646-CF|
|Human ADM ORF mammalian expression plasmid, C-His tag||HG11646-CH|
|Human ADM ORF mammalian expression plasmid, C-Myc tag||HG11646-CM|
|Human ADM ORF mammalian expression plasmid, C-HA tag||HG11646-CY|
|Human ADM Gene cDNA clone plasmid||HG11646-M|
|Human ADM ORF mammalian expression plasmid, N-Flag tag||HG11646-NF|
|Human ADM ORF mammalian expression plasmid, N-His tag||HG11646-NH|
|Human ADM ORF mammalian expression plasmid, N-Myc tag||HG11646-NM|
|Human ADM ORF mammalian expression plasmid, N-HA tag||HG11646-NY|
|Human ADM natural ORF mammalian expression plasmid||HG11646-UT|
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Adrenomedullin consists of 52 amino acids and is a member of the adrenomedullin family. It s a a hypotensive peptide and has 1 intramolecular disulfide bond. It seems that adrenomedullin has a slight homology with the calcitonin gene-related peptide. Adrenomedullin has a highly expression in pheochromocytoma and adrenal medulla. It also can be detected in lung, ventricle and kidney tissues. Adrenomedullin and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, adrenomedullin is diuretic and natriuretic, and both adrenomedullin and PAMP inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels. It is believed that adrenomedullin functions through combinations of the calcitonin receptor like receptor and receptor activity-modifying proteins complexes, as well as CGRP receptors.