|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human LRRN3 ORF mammalian expression plasmid, C-GFPSpark tag||HG11610-ACG|
|Human LRRN3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11610-ACR|
|Human LRRN3 ORF mammalian expression plasmid, C-Flag tag||HG11610-CF|
|Human LRRN3 ORF mammalian expression plasmid, C-His tag||HG11610-CH|
|Human LRRN3 ORF mammalian expression plasmid, C-Myc tag||HG11610-CM|
|Human LRRN3 ORF mammalian expression plasmid, C-HA tag||HG11610-CY|
|Human LRRN3 Gene cDNA clone plasmid||HG11610-M|
|Human LRRN3 ORF mammalian expression plasmid, N-Flag tag||HG11610-NF|
|Human LRRN3 ORF mammalian expression plasmid, N-His tag||HG11610-NH|
|Human LRRN3 ORF mammalian expression plasmid, N-Myc tag||HG11610-NM|
|Human LRRN3 ORF mammalian expression plasmid, N-HA tag||HG11610-NY|
|Human LRRN3 natural ORF mammalian expression plasmid||HG11610-UT|
|Learn more about expression Vectors|
Leucine-rich repeat neuronal protein 3, also known as neuronal leucine-rich repeat protein 3 (NLRR-3), is a member of leucine-rich (LRR) family whose members have significant functions in neural development. Leucine-rich repeats are short sequence motifs present in a number of proteins with diverse functions and cellular locations. All proteins containing these repeats are thought to be involved in protein-protein interactions. The crystal structure of ribonuclease inhibitor protein has revealed that leucine-rich repeats correspond to β-α structural units. These units are arranged so that they form a parallel β-sheet with one surface exposed to solvent, so that the protein acquires an unusual, non-globular shape. These two features may be responsible for the protein-binding functions of proteins containing leucine-rich repeats. LRRN3 plays an important role in cerebellum postnatal development. In a unilateral cortical injury cerebral cortex, NLRR-3 mRNA increased in layers 2-3 which suggests that NLRR-3 may be an important component of the pathophysiological response to brain injury.