|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human AKR1A1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11607-ACG|
|Human AKR1A1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11607-ACR|
|Human AKR1A1 ORF mammalian expression plasmid, N-GFPSpark tag||HG11607-ANG|
|Human AKR1A1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11607-ANR|
|Human AKR1A1 ORF mammalian expression plasmid, C-Flag tag||HG11607-CF|
|Human AKR1A1 ORF mammalian expression plasmid, C-His tag||HG11607-CH|
|Human AKR1A1 ORF mammalian expression plasmid, C-Myc tag||HG11607-CM|
|Human AKR1A1 ORF mammalian expression plasmid, C-HA tag||HG11607-CY|
|Human AKR1A1 Gene cDNA clone plasmid||HG11607-M|
|Human AKR1A1 ORF mammalian expression plasmid, N-Flag tag||HG11607-NF|
|Human AKR1A1 ORF mammalian expression plasmid, N-His tag||HG11607-NH|
|Human AKR1A1 ORF mammalian expression plasmid, N-Myc tag||HG11607-NM|
|Human AKR1A1 ORF mammalian expression plasmid, N-HA tag||HG11607-NY|
|Human AKR1A1 natural ORF mammalian expression plasmid||HG11607-UT|
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Aldehyde reductase (AKR1A1) is a member of the aldo-keto reductase superfamily, which consists of more than 40 known enzymes and proteins that includes variety of monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase. Aldehyde reductase has wide substrate specificities for carbonyl compounds. These enzymes are implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Aldehyde reductase possess a structure with a beta-alpha-beta fold which contains a novel NADP-binding motif. The binding site is located in a large, deep, elliptical pocket in the C-terminal end of the beta sheet, the substrate being bound in an extended conformation. This binding is more similar to FAD- than to NAD(P)-binding oxidoreductases. AKR1A1 is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue.