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Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-tagged

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AARSD1cDNA Clone Product Information
Gene_bank_ref_id:BC004172
cDNA Size:1578
cDNA Description:ORF Clone of Homo sapiens PTGES3L-AARSD1 readthrough DNA.
Gene Synonym:MGC2744, AARSD1
Species:Human
Vector:pCMV3-C-FLAG
Restriction Site:
Tag Sequence:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequence Description:
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pCMV3-C-FLAG Vector Information
 
Vector Name pCMV3-C-FLAG
Vector Size 6158bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag FLAG
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV3-C-FLAG Physical Map
Schematic of pCMV3-C-FLAG Multiple Cloning Sites

FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-tagged on other vectors
Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG14280-ACG$345
Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG14280-ACR$345
Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG14280-ANG$345
Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG14280-ANR$345
Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG14280-CF$315
Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG14280-CH$315
Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG14280-CM$315
Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG14280-CY$315
Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone)HG14280-G$195
Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG14280-NF$315
Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG14280-NH$315
Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG14280-NM$315
Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG14280-NY$315
Human PTGES3L-AARSD1 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG14280-UT$315
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Background

AARSD1 belongs to the class-II aminoacyl-tRNA synthetase family, Alax-L subfamily. AARSD1 binds 1 zinc ion per subunit functions in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala). Four transcript variants have been described for AARSD1: NM_025267.3, NM_001136042.2, NM_001142653.1 and NM_001142654.1. It has been determined that the latter two variants represent a distinct upstream locus, which is now represented by GeneID:100885848 (PTGES3L), while the former two variants represent readthrough transcripts between PTGES3L and this locus (AARSD1). The readthrough locus (PTGES3L-AARSD1) is now represented by GeneID:100885850.

References
  • Tatham MH, et al. (2011) Comparative proteomic analysis identifies a role for SUMO in protein quality control. Oncogene. 19(17):2120-8.
  • Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40.
  • Udeshi ND, et al. (2012) Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. Mol Cell Proteomics. 11(5):148-59.
  • Size / Price
    Catalog:HG14280-CF
    List Price: $315.00  (Save $0.00)
    Price:$315.00      [How to order]
    Availability2-3 weeks
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