|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human STK40 ORF mammalian expression plasmid, C-GFPSpark tag||HG11597-ACG|
|Human STK40 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11597-ACR|
|Human STK40 ORF mammalian expression plasmid, N-GFPSpark tag||HG11597-ANG|
|Human STK40 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11597-ANR|
|Human STK40 ORF mammalian expression plasmid, C-Flag tag||HG11597-CF|
|Human STK40 ORF mammalian expression plasmid, C-His tag||HG11597-CH|
|Human STK40 ORF mammalian expression plasmid, C-Myc tag||HG11597-CM|
|Human STK40 ORF mammalian expression plasmid, C-HA tag||HG11597-CY|
|Human STK40 Gene cDNA clone plasmid||HG11597-M|
|Human STK40 ORF mammalian expression plasmid, N-Flag tag||HG11597-NF|
|Human STK40 ORF mammalian expression plasmid, N-His tag||HG11597-NH|
|Human STK40 ORF mammalian expression plasmid, N-Myc tag||HG11597-NM|
|Human STK40 ORF mammalian expression plasmid, N-HA tag||HG11597-NY|
|Human STK40 natural ORF mammalian expression plasmid||HG11597-UT|
|Learn more about expression Vectors|
STK40 localized to both the cytoplasm and the nucleus. It is ubiquitously expressed. Mechanistically, Stk40 interacts with Rcn2, which also activates Erk1/2 to induce ExEn specification in mouse ESCs. Stk40 is able to activate the Erk/MAPK pathway and induce extraembryonic-endoderm (ExEn) differentiation in mouse ESCs. Interestingly, cells overexpressing Stk40 exclusively contribute to the ExEn layer of chimeric embryos when injected into host blastocysts. In contrast, deletion of Stk40 in ESCs markedly reduces ExEn differentiation in vitro. STK40 has a central serine/threonine protein kinase domain and is homologous to TRB-3, a protein that regulates activation of MAP kinases and inhibits NFκB-mediated gene transcription. Similarly, overexpression of STK40 inhibits NFκB activation triggered by TNF and also inhibits p53-mediated transcription. There are four named isoforms of STK40 that are produced as a result of alternative splicing.