|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human LCN1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11583-ACG|
|Human LCN1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11583-ACR|
|Human LCN1 ORF mammalian expression plasmid, C-Flag tag||HG11583-CF|
|Human LCN1 ORF mammalian expression plasmid, C-His tag||HG11583-CH|
|Human LCN1 ORF mammalian expression plasmid, C-Myc tag||HG11583-CM|
|Human LCN1 ORF mammalian expression plasmid, C-HA tag||HG11583-CY|
|Human LCN1 Gene cDNA clone plasmid||HG11583-M|
|Human LCN1 ORF mammalian expression plasmid, N-Flag tag||HG11583-NF|
|Human LCN1 ORF mammalian expression plasmid, N-His tag||HG11583-NH|
|Human LCN1 ORF mammalian expression plasmid, N-Myc tag||HG11583-NM|
|Human LCN1 ORF mammalian expression plasmid, N-HA tag||HG11583-NY|
|Human LCN1 natural ORF mammalian expression plasmid||HG11583-UT|
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Lipocalin-1, also known as Von Ebner gland protein, VEG protein, Tear prealbumin, VEGP, Tear lipocalin and LCN1, is a secreted protein which belongs to the calycin superfamily and Lipocalin family. Human Lipocalin-1 / VEGP was originally described as a major protein of human tear fluid, which was thought to be tear specific. Lipocalin-1 / VEGP is identical with lingual von Ebner's gland protein, and is also produced in prostate, nasal mucosa and tracheal mucosa. Homologous proteins have been found in rat, pig and probably dog and horse. Lipocalin-1 / VEGP is an unusual lipocalin member, because of its high promiscuity for relative insoluble lipids and binding characteristics that differ from other members. Lipocalin-1 / VEGP acts as the principal lipid binding protein in tear fluid, a more general physiological function has to be proposed due to its wide distribution and properties. Lipocalin-1 / VEGP would be ideally suited for scavenging of lipophilic, potentially harmful substances and thus might act as a general protection factor of epithelia. Lipocalin-1 / LCN1 could play a role in taste reception. It could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Lipocalin-1 / LCN1 can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. It exhibits an extremely wide ligand pocket.