|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ING4 ORF mammalian expression plasmid, C-GFPSpark tag||HG14264-ACG|
|Human ING4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14264-ACR|
|Human ING4 ORF mammalian expression plasmid, N-GFPSpark tag||HG14264-ANG|
|Human ING4 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14264-ANR|
|Human ING4 ORF mammalian expression plasmid, C-Flag tag||HG14264-CF|
|Human ING4 ORF mammalian expression plasmid, C-His tag||HG14264-CH|
|Human ING4 ORF mammalian expression plasmid, C-Myc tag||HG14264-CM|
|Human ING4 ORF mammalian expression plasmid, C-HA tag||HG14264-CY|
|Human ING4 Gene cDNA clone plasmid||HG14264-G|
|Human ING4 ORF mammalian expression plasmid, N-Flag tag||HG14264-NF|
|Human ING4 ORF mammalian expression plasmid, N-His tag||HG14264-NH|
|Human ING4 ORF mammalian expression plasmid, N-Myc tag||HG14264-NM|
|Human ING4 ORF mammalian expression plasmid, N-HA tag||HG14264-NY|
|Human ING4 natural ORF mammalian expression plasmid||HG14264-UT|
|Learn more about expression Vectors|
ING4 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. ING4 contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. ING4 protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in the TP53-dependent regulatory pathway. ING4 is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. ING4 may also inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation.