|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human NLK ORF mammalian expression plasmid, C-GFPSpark tag||HG11573-ACG|
|Human NLK ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11573-ACR|
|Human NLK ORF mammalian expression plasmid, N-GFPSpark tag||HG11573-ANG|
|Human NLK ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11573-ANR|
|Human NLK ORF mammalian expression plasmid, C-Flag tag||HG11573-CF|
|Human NLK ORF mammalian expression plasmid, C-His tag||HG11573-CH|
|Human NLK ORF mammalian expression plasmid, C-Myc tag||HG11573-CM|
|Human NLK ORF mammalian expression plasmid, C-HA tag||HG11573-CY|
|Human NLK Gene cDNA clone plasmid||HG11573-G|
|Human NLK ORF mammalian expression plasmid, N-Flag tag||HG11573-NF|
|Human NLK ORF mammalian expression plasmid, N-His tag||HG11573-NH|
|Human NLK ORF mammalian expression plasmid, N-Myc tag||HG11573-NM|
|Human NLK ORF mammalian expression plasmid, N-HA tag||HG11573-NY|
|Human NLK natural ORF mammalian expression plasmid||HG11573-UT|
|Learn more about expression Vectors|
Nemo-like kinase contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and MAP kinase subfamily. It also contains a TQE activation loop motif in which autophosphorylation of the threonine residue (Thr-298) is sufficient for kinase activation. As a serine/threonine-protein kinase, nemo-like kinase regulates a number of transcription factors with key roles in cell fate determination. It is a positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Nemo-like kinase also is a negative regulator of the canonical Wnt/beta-catenin signaling pathway.