|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CSNK1G1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11569-ACG|
|Human CSNK1G1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11569-ACR|
|Human CSNK1G1 ORF mammalian expression plasmid, N-GFPSpark tag||HG11569-ANG|
|Human CSNK1G1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11569-ANR|
|Human CSNK1G1 ORF mammalian expression plasmid, C-Flag tag||HG11569-CF|
|Human CSNK1G1 ORF mammalian expression plasmid, C-His tag||HG11569-CH|
|Human CSNK1G1 ORF mammalian expression plasmid, C-Myc tag||HG11569-CM|
|Human CSNK1G1 ORF mammalian expression plasmid, C-HA tag||HG11569-CY|
|Human CSNK1G1 Gene cDNA clone plasmid||HG11569-M|
|Human CSNK1G1 ORF mammalian expression plasmid, N-Flag tag||HG11569-NF|
|Human CSNK1G1 ORF mammalian expression plasmid, N-His tag||HG11569-NH|
|Human CSNK1G1 ORF mammalian expression plasmid, N-Myc tag||HG11569-NM|
|Human CSNK1G1 ORF mammalian expression plasmid, N-HA tag||HG11569-NY|
|Human CSNK1G1 natural ORF mammalian expression plasmid||HG11569-UT|
|Learn more about expression Vectors|
Casein kinase I isoform gamma-1, also known as CSNK1G1, is a member of the protein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. The casein kinase I family of protein kinases are serine / threonine-selective enzymes that function as regulators of signal transduction pathways in most eukaryotic cell types. Casein has been used as a substrate since the earliest days of research on protein phosphorylation. Casein kinase activity associated with the endoplasmic reticulum of mammary glands was first characterized in 1974 and its activity was shown to not depend on cyclic AMP. The CKI family of monomeric serine–threonine protein kinases is found in eukaryotic organisms from yeast to human. Mammals have seven family members: alpha, beta 1, gamma 1, gamma 2, gamma 3, delta, and epsilon. The family members have the highest homology in their kinase domains (53%–98% identical) and differ from most other protein kinases by the presence of the sequence S-I-N instead of A-P-E in kinase domain VIII. The CKI family members appear to have similar substrate specificity and substrate selection is thought to be regulated via subcellular localization and docking sites in specific substrates.