|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ATP1B1 ORF mammalian expression plasmid, C-GFPSpark tag||HG14255-ACG|
|Human ATP1B1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14255-ACR|
|Human ATP1B1 ORF mammalian expression plasmid, C-Flag tag||HG14255-CF|
|Human ATP1B1 ORF mammalian expression plasmid, C-His tag||HG14255-CH|
|Human ATP1B1 ORF mammalian expression plasmid, C-Myc tag||HG14255-CM|
|Human ATP1B1 ORF mammalian expression plasmid, C-HA tag||HG14255-CY|
|Human ATP1B1 Gene cDNA clone plasmid||HG14255-G|
|Human ATP1B1 ORF mammalian expression plasmid, N-Flag tag||HG14255-NF|
|Human ATP1B1 ORF mammalian expression plasmid, N-His tag||HG14255-NH|
|Human ATP1B1 ORF mammalian expression plasmid, N-Myc tag||HG14255-NM|
|Human ATP1B1 ORF mammalian expression plasmid, N-HA tag||HG14255-NY|
|Human ATP1B1 natural ORF mammalian expression plasmid||HG14255-UT|
|Learn more about expression Vectors|
ATP1B1 belongs to the family of Na+/K+ and H+/K+-ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. ATP1B1 is a subunit of Na+/K+-ATPase. Na+/K+-ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. Na+/K+-ATPase is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). ATP1B1 regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. ATP1B1 is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane.