|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CST1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11568-ACG|
|Human CST1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11568-ACR|
|Human CST1 ORF mammalian expression plasmid, C-Flag tag||HG11568-CF|
|Human CST1 ORF mammalian expression plasmid, C-His tag||HG11568-CH|
|Human CST1 ORF mammalian expression plasmid, C-Myc tag||HG11568-CM|
|Human CST1 ORF mammalian expression plasmid, C-HA tag||HG11568-CY|
|Human CST1 Gene cDNA clone plasmid||HG11568-M|
|Human CST1 ORF mammalian expression plasmid, N-Flag tag||HG11568-NF|
|Human CST1 ORF mammalian expression plasmid, N-His tag||HG11568-NH|
|Human CST1 ORF mammalian expression plasmid, N-Myc tag||HG11568-NM|
|Human CST1 ORF mammalian expression plasmid, N-HA tag||HG11568-NY|
|Human CST1 natural ORF mammalian expression plasmid||HG11568-UT|
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Cystatin-SN, also known as Cystain-SA-I, Cystatin-1, Salivary cystatin-SA-1 and CST1, is a secreted protein which belongs to the cystatin family. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. Cystatin-SN / CST1 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Cystatin-SN / CST1 is also expressed in saliva, tears, urine and seminal fluid. Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Human salivary cystatins include Cystatin-S, Cystatin-S1, Cystatin-S2, Cystatin- SA, Cystatin-SN, Cystatin-C and Cystatin-D. Cystatin-SN is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin-S, although both inhibit ficin equally well.