|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CST2 ORF mammalian expression plasmid, C-GFPSpark tag||HG11567-ACG|
|Human CST2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11567-ACR|
|Human CST2 ORF mammalian expression plasmid, C-Flag tag||HG11567-CF|
|Human CST2 ORF mammalian expression plasmid, C-His tag||HG11567-CH|
|Human CST2 ORF mammalian expression plasmid, C-Myc tag||HG11567-CM|
|Human CST2 ORF mammalian expression plasmid, C-HA tag||HG11567-CY|
|Human CST2 Gene cDNA clone plasmid||HG11567-M|
|Human CST2 ORF mammalian expression plasmid, N-Flag tag||HG11567-NF|
|Human CST2 ORF mammalian expression plasmid, N-His tag||HG11567-NH|
|Human CST2 ORF mammalian expression plasmid, N-Myc tag||HG11567-NM|
|Human CST2 ORF mammalian expression plasmid, N-HA tag||HG11567-NY|
|Human CST2 natural ORF mammalian expression plasmid||HG11567-UT|
|Learn more about expression Vectors|
Cystatin-SA, also known as Cystatin-2, Cystatin-S5 and CST2, is a secreted protein which belongs to the cystatin family. Cystatin-2 / CST2 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). It is also expressed in submandibular gland and parotid gland. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The CST1, CST2, CST4, and CST5 are expressed in differential, tissue-specific patterns. Expression of CST2 and CST5 is restricted to the submandibular and parotid glands, while CST1 and CST4 are expressed in these tissues and in the lacrimal gland.