|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human NETO1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11562-ACG|
|Human NETO1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11562-ACR|
|Human NETO1 ORF mammalian expression plasmid, C-Flag tag||HG11562-CF|
|Human NETO1 ORF mammalian expression plasmid, C-His tag||HG11562-CH|
|Human NETO1 ORF mammalian expression plasmid, C-Myc tag||HG11562-CM|
|Human NETO1 ORF mammalian expression plasmid, C-HA tag||HG11562-CY|
|Human NETO1 Gene cDNA clone plasmid||HG11562-M|
|Human NETO1 ORF mammalian expression plasmid, N-Flag tag||HG11562-NF|
|Human NETO1 ORF mammalian expression plasmid, N-His tag||HG11562-NH|
|Human NETO1 ORF mammalian expression plasmid, N-Myc tag||HG11562-NM|
|Human NETO1 ORF mammalian expression plasmid, N-HA tag||HG11562-NY|
|Human NETO1 natural ORF mammalian expression plasmid||HG11562-UT|
|Learn more about expression Vectors|
Neuropilin tolloid-like 1 (NETO1), a complement C1r/C1s, Uegf, Bmp1 (CUB) domain-containing transmembrane protein, is a novel component of the NMDAR complex critical for maintaining the abundance of NR2A-containing NMDARs in the postsynaptic density. The N-methyl-D-aspartate receptor (NMDAR), a major excitatory ligand-gated ion channel in the central nervous system (CNS), is a principal mediator of synaptic plasticity. Both NETO1 and NETO2 share an identical and unique domain structure thus representing a novel subfamily of CUB- and LDLa-containing proteins. The cytoplasmic domains of NETO1 and NETO2 are not homologous to other known protein sequences but contain a conserved FXNPXY-like motif, which is essential for the internalization of clathrin coated pits during endocytosis or alternatively, may be implicated in intracellular signaling pathways. NETO1 and NETO2, have marked effects on receptor properties, increasing further the potential diversity of Kainate receptors (KARs) functional properties. NETO1 involves in the development and/or maintenance of neuronal circuitry. NETO1 regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.