|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ICOSLG ORF mammalian expression plasmid, C-GFPSpark tag||HG11559-ACG|
|Human ICOSLG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11559-ACR|
|Human ICOSLG ORF mammalian expression plasmid, C-Flag tag||HG11559-CF|
|Human ICOSLG ORF mammalian expression plasmid, C-His tag||HG11559-CH|
|Human ICOSLG ORF mammalian expression plasmid, C-Myc tag||HG11559-CM|
|Human ICOSLG ORF mammalian expression plasmid, C-HA tag||HG11559-CY|
|Human ICOSLG ORF mammalian expression plasmid, N-Flag tag||HG11559-NF|
|Human ICOSLG ORF mammalian expression plasmid, N-His tag||HG11559-NH|
|Human ICOSLG ORF mammalian expression plasmid, N-Myc tag||HG11559-NM|
|Human ICOSLG ORF mammalian expression plasmid, N-HA tag||HG11559-NY|
|Human ICOSLG natural ORF mammalian expression plasmid||HG11559-UT|
|Learn more about expression Vectors|
Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. Present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production and germinal center formation.