|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human VRK1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11555-ACG|
|Human VRK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11555-ACR|
|Human VRK1 ORF mammalian expression plasmid, N-GFPSpark tag||HG11555-ANG|
|Human VRK1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11555-ANR|
|Human VRK1 ORF mammalian expression plasmid, C-Flag tag||HG11555-CF|
|Human VRK1 ORF mammalian expression plasmid, C-His tag||HG11555-CH|
|Human VRK1 ORF mammalian expression plasmid, C-Myc tag||HG11555-CM|
|Human VRK1 ORF mammalian expression plasmid, C-HA tag||HG11555-CY|
|Human VRK1 Gene cDNA clone plasmid||HG11555-M|
|Human VRK1 ORF mammalian expression plasmid, N-Flag tag||HG11555-NF|
|Human VRK1 ORF mammalian expression plasmid, N-His tag||HG11555-NH|
|Human VRK1 ORF mammalian expression plasmid, N-Myc tag||HG11555-NM|
|Human VRK1 ORF mammalian expression plasmid, N-HA tag||HG11555-NY|
|Human VRK1 natural ORF mammalian expression plasmid||HG11555-UT|
|Learn more about expression Vectors|
VRK1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. Serine/threonine protein kinases are tumor suppressor that controls the activity of AMP-activated protein kinase family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. VRK1 contains 1 protein kinase domain and localizes to the nucleus. VRK1 gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. As a serine/threonine kinase, VRK1 phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1), also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy.