|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human NEK7 ORF mammalian expression plasmid, C-GFPSpark tag||HG11534-ACG|
|Human NEK7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11534-ACR|
|Human NEK7 ORF mammalian expression plasmid, N-GFPSpark tag||HG11534-ANG|
|Human NEK7 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11534-ANR|
|Human NEK7 ORF mammalian expression plasmid, C-Flag tag||HG11534-CF|
|Human NEK7 ORF mammalian expression plasmid, C-His tag||HG11534-CH|
|Human NEK7 ORF mammalian expression plasmid, C-Myc tag||HG11534-CM|
|Human NEK7 ORF mammalian expression plasmid, C-HA tag||HG11534-CY|
|Human NEK7 Gene cDNA clone plasmid||HG11534-M|
|Human NEK7 ORF mammalian expression plasmid, N-Flag tag||HG11534-NF|
|Human NEK7 ORF mammalian expression plasmid, N-His tag||HG11534-NH|
|Human NEK7 ORF mammalian expression plasmid, N-Myc tag||HG11534-NM|
|Human NEK7 ORF mammalian expression plasmid, N-HA tag||HG11534-NY|
|Human NEK7 natural ORF mammalian expression plasmid||HG11534-UT|
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NIMA (never in mitosis gene a)-related kinase 7, NEK7 belongs to the NIMA subfamily, NEK Ser/Thr protein kinase family, protein kinase superfamily. NEKs (NIMA-related kinases) are mammalian serine/threonine (Ser/Thr) protein kinases structurally related to Aspergillus NIMA (Never in Mitosis, gene A), which plays essential roles in mitotic signaling. NEKs share an amino-terminal catalytic domain related to NIMA, an Aspergillus kinase involved in the control of several aspects of mitosis, and divergent carboxyl-terminal tails of varying length. NEKs are commonly referred to as mitotic kinases, although a definitive in vivo verification of this definition is largely missing. Reduction in the activity of NEK7 or its close paralog, NEK6, has previously been shown to arrest cells in mitosis, mainly at metaphase. NEK7 is a regulator of cell division, and reveal it as an essential component for mammalian growth and survival. The intimate connection between tetraploidy, aneuploidy and cancer development suggests that NEK7 deregulation can induce oncogenesis. The endogenous NEK7 protein is enriched at the centrosome in a microtubule-independent manner. Overexpression of wt or kinase-defective NEK7 resulted in cells of rounder appearance, and higher proportions of multinuclear and apoptotic cells.