|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TSPAN31 ORF mammalian expression plasmid, C-GFPSpark tag||HG14231-ACG|
|Human TSPAN31 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14231-ACR|
|Human TSPAN31 ORF mammalian expression plasmid, C-Flag tag||HG14231-CF|
|Human TSPAN31 ORF mammalian expression plasmid, C-His tag||HG14231-CH|
|Human TSPAN31 ORF mammalian expression plasmid, C-Myc tag||HG14231-CM|
|Human TSPAN31 ORF mammalian expression plasmid, C-HA tag||HG14231-CY|
|Human TSPAN31 Gene cDNA clone plasmid||HG14231-G|
|Human TSPAN31 ORF mammalian expression plasmid, N-Flag tag||HG14231-NF|
|Human TSPAN31 ORF mammalian expression plasmid, N-His tag||HG14231-NH|
|Human TSPAN31 ORF mammalian expression plasmid, N-Myc tag||HG14231-NM|
|Human TSPAN31 ORF mammalian expression plasmid, N-HA tag||HG14231-NY|
|Human TSPAN31 natural ORF mammalian expression plasmid||HG14231-UT|
|Learn more about expression Vectors|
TSPAN31 is a member of the transmembrane 4 superfamily. Most members of this family are cell-surface proteins that are characterized by the presence of four hydrophobic domains. They mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TSPAN31 is thought to be involved in growth-related cellular processes. This gene is associated with tumorigenesis and osteosarcoma.