|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IVD ORF mammalian expression plasmid, C-GFPSpark tag||HG14206-ACG|
|Human IVD ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14206-ACR|
|Human IVD ORF mammalian expression plasmid, N-GFPSpark tag||HG14206-ANG|
|Human IVD ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14206-ANR|
|Human IVD ORF mammalian expression plasmid, C-Flag tag||HG14206-CF|
|Human IVD ORF mammalian expression plasmid, C-His tag||HG14206-CH|
|Human IVD ORF mammalian expression plasmid, C-Myc tag||HG14206-CM|
|Human IVD ORF mammalian expression plasmid, C-HA tag||HG14206-CY|
|Human IVD Gene cDNA clone plasmid||HG14206-G|
|Human IVD ORF mammalian expression plasmid, N-Flag tag||HG14206-NF|
|Human IVD ORF mammalian expression plasmid, N-His tag||HG14206-NH|
|Human IVD ORF mammalian expression plasmid, N-Myc tag||HG14206-NM|
|Human IVD ORF mammalian expression plasmid, N-HA tag||HG14206-NY|
|Human IVD natural ORF mammalian expression plasmid||HG14206-UT|
|Learn more about expression Vectors|
Isovaleryl-CoA dehydrogenase, also known as IVD, plays an essential role in processing proteins obtained from the diet. The body breaks down proteins from food into smaller parts called amino acids. Amino acids can be further processed to provide energy for growth and development. Isovaleryl-CoA dehydrogenase helps process a particular amino acid called leucine. Specifically, isovaleryl-CoA dehydrogenase is responsible for the third step in the breakdown of leucine. This step is a chemical reaction that converts a molecule called isovaleryl-CoA to another molecule, 3-methylcrotonyl-CoA. Additional chemical reactions convert 3-methylcrotonyl-CoA into molecules that are used for energy.