|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CTRC ORF mammalian expression plasmid, C-GFPSpark tag||HG11456-ACG|
|Human CTRC ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11456-ACR|
|Human CTRC ORF mammalian expression plasmid, N-GFPSpark tag||HG11456-ANG|
|Human CTRC ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11456-ANR|
|Human CTRC ORF mammalian expression plasmid, C-Flag tag||HG11456-CF|
|Human CTRC ORF mammalian expression plasmid, C-His tag||HG11456-CH|
|Human CTRC ORF mammalian expression plasmid, C-Myc tag||HG11456-CM|
|Human CTRC ORF mammalian expression plasmid, C-HA tag||HG11456-CY|
|Human CTRC Gene cDNA clone plasmid||HG11456-M|
|Human CTRC ORF mammalian expression plasmid, N-Flag tag||HG11456-NF|
|Human CTRC ORF mammalian expression plasmid, N-His tag||HG11456-NH|
|Human CTRC ORF mammalian expression plasmid, N-Myc tag||HG11456-NM|
|Human CTRC ORF mammalian expression plasmid, N-HA tag||HG11456-NY|
|Human CTRC natural ORF mammalian expression plasmid||HG11456-UT|
|Learn more about expression Vectors|
Chymotrypsin C (abbreviated for CTRC), also known as caldecrin or elastase4, is a digestive enzyme of the peptidase S1 family. This enzyme is synthesized as an inactivate chymotrypsinogen. On cleavage by trypsin into two parts that activate each other by removing two small peptides in a trans-proteolysis, chymotrypsin C produced. N-linked glycosylation of human CTRC is required for efficient folding and secretion, however, the N-linked glycan is unimportant for enzyme activity or inhibitor binding. It has been proposed that CTRC is a key regulator of digestive zymogen activation and a physiological co-activator of digestive carboxypeptidases proCPA1 and proCPA2. Mutations that abolish activity or secretion of CTRC increase the risk for chronic pancreatitis. It's speculated that CTRC might regulate pancreatic cancer cell migration in relation to cytokeratin 18 expression. The pancreatic cancer cell migration ability was downregulated in pancreatic cancer Aspc-1 cells that overexpressed CTRC, whereas the cell migration ability was upregulated in Aspc-1 cells in which CTRC was suppressed.