|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human HSP90AA1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11445-ACG|
|Human HSP90AA1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11445-ACR|
|Human HSP90AA1 ORF mammalian expression plasmid, N-GFPSpark tag||HG11445-ANG|
|Human HSP90AA1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11445-ANR|
|Human HSP90AA1 ORF mammalian expression plasmid, C-Flag tag||HG11445-CF|
|Human HSP90AA1 ORF mammalian expression plasmid, C-His tag||HG11445-CH|
|Human HSP90AA1 ORF mammalian expression plasmid, C-Myc tag||HG11445-CM|
|Human HSP90AA1 ORF mammalian expression plasmid, C-HA tag||HG11445-CY|
|Human HSP90AA1 Gene cDNA clone plasmid||HG11445-G|
|Human HSP90AA1 ORF mammalian expression plasmid, N-Flag tag||HG11445-NF|
|Human HSP90AA1 ORF mammalian expression plasmid, N-His tag||HG11445-NH|
|Human HSP90AA1 ORF mammalian expression plasmid, N-Myc tag||HG11445-NM|
|Human HSP90AA1 ORF mammalian expression plasmid, N-HA tag||HG11445-NY|
|Human HSP90AA1 natural ORF mammalian expression plasmid||HG11445-UT|
|Learn more about expression Vectors|
Heat shock protein 90 (90 kDa heat-shock protein, HSP90) is a molecular chaperone involved in the trafficking of proteins in the cell. It is a remarkably versatile protein involved in the stress response and in normal homoeostatic control mechanisms. HSP90 interacts with 'client proteins', including protein kinases, transcription factors and others, and either facilitates their stabilization and activation or directs them for proteasomal degradation. By this means, HSP90 displays a multifaceted ability to influence signal transduction, chromatin remodelling and epigenetic regulation, development and morphological evolution. HSP90 operates as a dimer in a conformational cycle driven by ATP binding and hydrolysis at the N-terminus. Disruption of HSP90 leads to client protein degradation and often cell death. Under stressful conditions, HSP90 stabilizes its client proteins and provides protection to the cell against cellular stressors such as in cancer cells. Especially, several oncoproteins act as HSP90 client proteins and tumor cells require higher HSP90 activity than normal cells to maintain their malignancy. For this reason, Hsp90 has emerged as a promising target for anti-cancer drug development.