|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human LILRB2 ORF mammalian expression plasmid, C-GFPSpark tag||HG14132-ACG|
|Human LILRB2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14132-ACR|
|Human LILRB2 ORF mammalian expression plasmid, C-Flag tag||HG14132-CF|
|Human LILRB2 ORF mammalian expression plasmid, C-His tag||HG14132-CH|
|Human LILRB2 ORF mammalian expression plasmid, C-Myc tag||HG14132-CM|
|Human LILRB2 ORF mammalian expression plasmid, C-HA tag||HG14132-CY|
|Human LILRB2 Gene cDNA clone plasmid||HG14132-G|
|Human LILRB2 ORF mammalian expression plasmid, N-Flag tag||HG14132-NF|
|Human LILRB2 ORF mammalian expression plasmid, N-His tag||HG14132-NH|
|Human LILRB2 ORF mammalian expression plasmid, N-Myc tag||HG14132-NM|
|Human LILRB2 ORF mammalian expression plasmid, N-HA tag||HG14132-NY|
|Human LILRB2 natural ORF mammalian expression plasmid||HG14132-UT|
|Learn more about expression Vectors|
ILT4, also known as LILRB2, is a member of the the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). ILT4 gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family. Multiple transcript variants encoding different isoforms have been found for ILT4 gene. ILT4 is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity.