|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PMP2 ORF mammalian expression plasmid, C-GFPSpark tag||HG11426-ACG|
|Human PMP2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11426-ACR|
|Human PMP2 ORF mammalian expression plasmid, N-GFPSpark tag||HG11426-ANG|
|Human PMP2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11426-ANR|
|Human PMP2 ORF mammalian expression plasmid, C-Flag tag||HG11426-CF|
|Human PMP2 ORF mammalian expression plasmid, C-His tag||HG11426-CH|
|Human PMP2 ORF mammalian expression plasmid, C-Myc tag||HG11426-CM|
|Human PMP2 ORF mammalian expression plasmid, C-HA tag||HG11426-CY|
|Human PMP2 Gene cDNA clone plasmid||HG11426-M|
|Human PMP2 ORF mammalian expression plasmid, N-Flag tag||HG11426-NF|
|Human PMP2 ORF mammalian expression plasmid, N-His tag||HG11426-NH|
|Human PMP2 ORF mammalian expression plasmid, N-Myc tag||HG11426-NM|
|Human PMP2 ORF mammalian expression plasmid, N-HA tag||HG11426-NY|
|Human PMP2 natural ORF mammalian expression plasmid||HG11426-UT|
|Learn more about expression Vectors|
Myelin P2 protein, also known as PMP2, is a cytosolic protein found primarily in peripheral nerves. It Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. PMP2 is a small, basic, and cytoplasmic lipid binding protein of peripheral myelin. It is similar in amino acid sequence and tertiary structure to fatty acid binding proteins found in the liver, adipocytes, and intestine, its expression is limited to the nervous system. PMP2 is detected only in myelin-producing cells of the central and peripheral nervous systems, the oligodendrocytes and Schwann cells, respectively. PMP2 may play a role in lipid transport protein in Schwann cells. It forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.