|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human B3GNT2 ORF mammalian expression plasmid, C-GFPSpark tag||HG11416-ACG|
|Human B3GNT2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11416-ACR|
|Human B3GNT2 ORF mammalian expression plasmid, C-Flag tag||HG11416-CF|
|Human B3GNT2 ORF mammalian expression plasmid, C-His tag||HG11416-CH|
|Human B3GNT2 ORF mammalian expression plasmid, C-Myc tag||HG11416-CM|
|Human B3GNT2 ORF mammalian expression plasmid, C-HA tag||HG11416-CY|
|Human B3GNT2 Gene cDNA clone plasmid||HG11416-M|
|Human B3GNT2 ORF mammalian expression plasmid, N-Flag tag||HG11416-NF|
|Human B3GNT2 ORF mammalian expression plasmid, N-His tag||HG11416-NH|
|Human B3GNT2 ORF mammalian expression plasmid, N-Myc tag||HG11416-NM|
|Human B3GNT2 ORF mammalian expression plasmid, N-HA tag||HG11416-NY|
|Human B3GNT2 natural ORF mammalian expression plasmid||HG11416-UT|
|Learn more about expression Vectors|
B3GNT2 belongs to the beta-1,3-N-acetylglucosaminyltransferase family. It is a type II transmembrane protein which prefers the substrate of lacto-N-neotetraose. Alternative splicing produced 2 isoforms of the human protein. B3GNT2 catalyzes the initiation and elongation of poly-N- acetyllactosamine chains. Enzymatic activities of some glycosyltransferases are markedly increased via complex formation with other transferases or cofactor proteins. B3GNT2 and beta3Gn-T8 can form a heterodimer in vitro and that the complex exhibits much higher enzymatic activity than either enzyme alone. It is found that up-regulation of beta3Gn-T8 in differentiated HL-60 cells may increases poly-N-acetyllactosamine chains by activating intrinsic B3GNT2.