|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FLRT1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11389-ACG|
|Human FLRT1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11389-ACR|
|Human FLRT1 ORF mammalian expression plasmid, C-Flag tag||HG11389-CF|
|Human FLRT1 ORF mammalian expression plasmid, C-His tag||HG11389-CH|
|Human FLRT1 ORF mammalian expression plasmid, C-Myc tag||HG11389-CM|
|Human FLRT1 ORF mammalian expression plasmid, C-HA tag||HG11389-CY|
|Human FLRT1 Gene cDNA clone plasmid||HG11389-M|
|Human FLRT1 ORF mammalian expression plasmid, Flag tag||HG11389-M-F|
|Human FLRT1 ORF mammalian expression plasmid, N-Flag tag||HG11389-NF|
|Human FLRT1 ORF mammalian expression plasmid, N-His tag||HG11389-NH|
|Human FLRT1 ORF mammalian expression plasmid, N-Myc tag||HG11389-NM|
|Human FLRT1 ORF mammalian expression plasmid, N-HA tag||HG11389-NY|
|Human FLRT1 natural ORF mammalian expression plasmid||HG11389-UT|
|Learn more about expression Vectors|
The three fibronectin leucine-rich repeat transmembrane (FLRT) proteins contain 10 leucine-rich repeats (LRR), a type III fibronectin (FN) domain, followed by the transmembrane region, and a short cytoplasmic tail. FLRT1 is expressed in kidney and brain, which is a target for tyrosine phosphorylation mediated by FGFR1 and implicate a non-receptor Src family kinase (SFK). All FLRTs can interact with FGFR1 and FLRTs can be induced by the activation of FGF signalling by FGF-2. The phosphorylation state of FLRT1, which is itself FGFR1 dependent, may play a critical role in the potentiation of FGFR1 signalling and may also depend on a SFK-dependent phosphorylation mechanism acting via the FGFR. This is consistent with an 'in vivo' role for FLRT1 regulation of FGF signalling via SFKs. Furthermore, the phosphorylation-dependent futile cycle mechanism controlling FGFR1 signalling is concurrently crucial for regulation of FLRT1-mediated neurite outgrowth. FLRT1, FLRT2 and FLRT3 are members of the fibronectin leucine rich transmembrane protein (FLRT) family. They may function in cell adhesion and/or receptor signalling. Their protein structures resemble small leucine-rich proteoglycans found in the extracellular matrix. FLRT3 shares 55% amino acid sequence identity with FLRT1.