|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human LRRTM4 ORF mammalian expression plasmid, C-GFPSpark tag||HG11387-ACG|
|Human LRRTM4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11387-ACR|
|Human LRRTM4 ORF mammalian expression plasmid, C-Flag tag||HG11387-CF|
|Human LRRTM4 ORF mammalian expression plasmid, C-His tag||HG11387-CH|
|Human LRRTM4 ORF mammalian expression plasmid, C-Myc tag||HG11387-CM|
|Human LRRTM4 ORF mammalian expression plasmid, C-HA tag||HG11387-CY|
|Human LRRTM4 Gene cDNA clone plasmid||HG11387-M|
|Human LRRTM4 ORF mammalian expression plasmid, N-Flag tag||HG11387-NF|
|Human LRRTM4 ORF mammalian expression plasmid, N-His tag||HG11387-NH|
|Human LRRTM4 ORF mammalian expression plasmid, N-Myc tag||HG11387-NM|
|Human LRRTM4 ORF mammalian expression plasmid, N-HA tag||HG11387-NY|
|Human LRRTM4 natural ORF mammalian expression plasmid||HG11387-UT|
|Learn more about expression Vectors|
Leucine-rich repeat transmembrane neuronal protein 4, also known as LRRTM4, is a single-pass type I membrane protein which belongs to the LRRTM family. LRRTM4 is expressed in the limb mesenchyme, neural tube, caudal mesoderm and in three distinct regions of the head. LRRTM4 may play a role in the development and maintenance of the vertebrate nervous system. Leucine-rich repeat containing proteins are involved in protein-protein interactions and they regulate numerous cellular events during nervous system development and disease. Human and mouse LRRTMs are highly conserved, and orthologous genes exist in other vertebrates but not in invertebrates. LRRTM mRNAs are predominantly expressed in the nervous system and that each LRRTM possesses a specific, partially nonoverlapping expression pattern. The structure and expression profile of LRRTM mRNAs suggest that they may have a role in the development and maintenance of the vertebrate nervous system. All LRRTMs, except LRRTM4, are located in the introns of different alpha-catenin genes, suggesting coevolution of these two gene families.