|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CD180 ORF mammalian expression plasmid, C-GFPSpark tag||HG11370-ACG|
|Human CD180 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11370-ACR|
|Human CD180 ORF mammalian expression plasmid, C-Flag tag||HG11370-CF|
|Human CD180 ORF mammalian expression plasmid, C-His tag||HG11370-CH|
|Human CD180 ORF mammalian expression plasmid, C-Myc tag||HG11370-CM|
|Human CD180 ORF mammalian expression plasmid, C-HA tag||HG11370-CY|
|Human CD180 Gene cDNA clone plasmid||HG11370-M|
|Human CD180 ORF mammalian expression plasmid, Flag tag||HG11370-M-F|
|Human CD180 ORF mammalian expression plasmid, His tag||HG11370-M-H|
|Human CD180 ORF mammalian expression plasmid, N-Flag tag||HG11370-NF|
|Human CD180 ORF mammalian expression plasmid, N-His tag||HG11370-NH|
|Human CD180 ORF mammalian expression plasmid, N-Myc tag||HG11370-NM|
|Human CD180 ORF mammalian expression plasmid, N-HA tag||HG11370-NY|
|Human CD180 natural ORF mammalian expression plasmid||HG11370-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD180, also known as RP105, is a B-cell surface molecule belonging to the family of pathogen receptors, Toll-like receptors (TLR). CD180 has an extracellular leucine-rich repeats and a short cytoplasmic tail. CD180 / RP105 interact with an extracellular molecule named MD1 and then together form the cell surface receptor complex RP105 / MD1 which induces B-cell activation in humans and mice, leading to proliferation and up-regulation of a costimulatory molecule, B7.2 / CD86. CD180 / RP105 also has a role in LPS response because B cells lacking RP105 show hyporesponsiveness to LPS.