|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human HCST ORF mammalian expression plasmid, C-GFPSpark tag||HG14063-ACG|
|Human HCST ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14063-ACR|
|Human HCST ORF mammalian expression plasmid, C-Flag tag||HG14063-CF|
|Human HCST ORF mammalian expression plasmid, C-His tag||HG14063-CH|
|Human HCST ORF mammalian expression plasmid, C-Myc tag||HG14063-CM|
|Human HCST ORF mammalian expression plasmid, C-HA tag||HG14063-CY|
|Human HCST Gene cDNA clone plasmid||HG14063-G|
|Human HCST ORF mammalian expression plasmid, N-Flag tag||HG14063-NF|
|Human HCST ORF mammalian expression plasmid, N-His tag||HG14063-NH|
|Human HCST ORF mammalian expression plasmid, N-Myc tag||HG14063-NM|
|Human HCST ORF mammalian expression plasmid, N-HA tag||HG14063-NY|
|Human HCST natural ORF mammalian expression plasmid||HG14063-UT|
|Learn more about expression Vectors|
HCST, also known as DAP10, belongs to the DAP10 family. It is a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. HCST may be part of the immune recognition receptor complex. This receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs). It may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif.