|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human SOCS3 ORF mammalian expression plasmid, C-GFPSpark tag||HG11315-ACG|
|Human SOCS3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11315-ACR|
|Human SOCS3 ORF mammalian expression plasmid, N-GFPSpark tag||HG11315-ANG|
|Human SOCS3 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Flag-tagged||HG11315-CF|
|Human SOCS3 ORF mammalian expression plasmid, C-His tag||HG11315-CH|
|Human SOCS3 ORF mammalian expression plasmid, C-Myc tag||HG11315-CM|
|Human SOCS3 ORF mammalian expression plasmid, C-HA tag||HG11315-CY|
|Human SOCS3 ORF mammalian expression plasmid, N-Flag tag||HG11315-NF|
|Human SOCS3 ORF mammalian expression plasmid, N-His tag||HG11315-NH|
|Human SOCS3 ORF mammalian expression plasmid, N-Myc tag||HG11315-NM|
|Human SOCS3 ORF mammalian expression plasmid, N-HA tag||HG11315-NY|
|Human SOCS3 natural ORF mammalian expression plasmid||HG11315-UT|
|Learn more about expression Vectors|
Suppressor of cytokine signaling 3, also known as SOCS-3, Cytokine-inducible SH2 protein 3, CIS-3, STAT-induced STAT inhibitor 3, SOCS3 and CIS3, is a protein which is widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. SOCS3 / CIS3 contains one SH2 domain and one SOCS box domain. SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 / CIS3 is involved in negative regulation of cytokines that signal through the JAK / STAT pathway. SOCS3 / CIS3 inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. SOCS3 / CIS3 suppresses fetal liver erythropoiesis. It regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. SOCS3 / CIS3 regulates IL-6 signaling. SOCS3 / CIS3 interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. SOCS3 / CIS3 could be used as a possible therapeutic agent for treating rheumatoid arthritis.