|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GPA33 ORF mammalian expression plasmid, C-GFPSpark tag||HG11277-ACG|
|Human GPA33 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11277-ACR|
|Human GPA33 ORF mammalian expression plasmid, C-Flag tag||HG11277-CF|
|Human GPA33 ORF mammalian expression plasmid, C-His tag||HG11277-CH|
|Human GPA33 ORF mammalian expression plasmid, C-Myc tag||HG11277-CM|
|Human GPA33 ORF mammalian expression plasmid, C-HA tag||HG11277-CY|
|Human GPA33 Gene cDNA clone plasmid||HG11277-M|
|Human GPA33 ORF mammalian expression plasmid, Flag tag||HG11277-M-F|
|Human GPA33 ORF mammalian expression plasmid, N-Flag tag||HG11277-NF|
|Human GPA33 ORF mammalian expression plasmid, N-His tag||HG11277-NH|
|Human GPA33 ORF mammalian expression plasmid, N-Myc tag||HG11277-NM|
|Human GPA33 ORF mammalian expression plasmid, N-HA tag||HG11277-NY|
|Human GPA33 natural ORF mammalian expression plasmid||HG11277-UT|
|Learn more about expression Vectors|
Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation. GPA33 has become a promising target of immunologic therapy strategies, but its biologic function and potential role in tumorigenesis are unknown. EpCAM protein and GPA33 mRNA expressions are specific and sensitive markers of Barrett's metaplasia (BM). GPA33 may also play a role in cell-cell recognition and signaling.